Modifying Enzymes
Abcam URB754, Monoacylglycerol lipase inhibitor, 50MG
MW 266.3 g/mol, Purity >98%. Reported potent and non-competitive monoacylglycerol lipase (MAGL) inhibitor (IC₅₀ = 200 nM). Inhibits FAAH (IC₅₀ = 32 μM) and binds weakly to the rat CB₁ receptor (IC₅₀ = 3.8 μM). Shows vasodilatory activity.
The product is subject to the following: Abcam Restricted Use Statement
Worthington Biochemical Corporation HYALURONIDASE PURIFIED 5KUHYA
Chromatographically purified. A dialyzed, lyophilized powder.
Abcam Amsacrine, DNA topoisomerase II inhibitor, 10MG
MW 393.5 Da, Purity >98%. DNA topoisomerase II inhibitor. Antineoplastic agent. Intercalating planar polycyclic aromatic molecule which is active against a number of tumours in mice. Active in vivo..
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Liberase(TM) TM Research Grade medium Thermolysin concentration |
Liberase(TM) TM Research Grade medium Thermolysin concentration |
Sigma Aldrich Fine Chemicals Biosciences Apyrase from potatoes, 9000-95-7, MFCD00130542, 500UN
Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein. Synonym: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase, Apyrase from potatoes. Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
Sigma Aldrich Fine Chemicals Biosciences Pectinase from Aspergillus aculeatus | 50 mL
Pectinase is an enzyme from Aspergillus aculeatus that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to conduct partial saccharification of sugars. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications. Pectinase, product P2611, has been used to treat leaf discs from fifth wild-type and sitiens leaves.
Takara Bio CRYONASE COLD-ACTIVE NUCLEASE
Enzyme; Cryonase Cold-active Nuclease; Takara; 10,000U (20 U/uLx500 uL); Source: Purified from E. coli; Purity: Protease activity is less than detection limit; Storage: -20 deg.C
Takara Bio TAQ HOT START PREMIX
PCR Reagents; Takara; Hot-Start Taq DNA Polymerase; 100rxns; reduces background, increased specificity, permits room-temperature reaction assembly
New England Biolabs, Inc. Instant Sticky-end Ligase Master Mix – 50 reactions
Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer. Specifically formulated to rapidly ligate cohesive-end (2-4 bp) substrates and improve transformation, the mix simplifies reaction set-up and provides an optimized ratio of enzyme and buffer components. No thawing of the master mix is required as it maintains a liquid state during storage at -20C and no incubation time is necessary to achieve ligation efficiencies sufficient for successful sub-cloning of sticky-end substrates. Just add the master mix to DNA with compatible ends, mix and transform; thereby reducing the valuable time needed for routine ligations. Ligations for subcloning can be carried out in small volumes with low concentrations, allowing users to conserve precious DNA samples, and be used directly to transform many strains of chemically competent E. coli without dilution.
Abcam NADP, Coenzyme, 1G
MW 787.37 g/mol, Purity >=93%. NADP and NADPH form a redox pair. NADP is the oxidised form of NADPH. NADP/NADPH is a coenzyme that supports redox reactions via the transport of electrons in a vast array of applications. Acts as a coenzyme couple in various cytochrome P450 systems and oxidase/reductase reaction systems.
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. SacI-HF – 2000 units
A high fidelity restriction endonuclease that recognizes the sequence G_AGCT^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. USER® Enzyme – 250 units
USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3 and 5 sides of the abasic site so that base-free deoxyribose is released.Applications of USER include:
- Directional RNA Seq
- NEBNext adaptor cleavage
- USER cloning
New England Biolabs, Inc. E.coli DNA Ligase – 200 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. T4 DNA Ligase – 100,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. T4 RNA Ligase 1 (ssRNA Ligase) – 5000 units
T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3' - 5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.